2,3,5-Triphenyltetrazolium chloride
2,3,5-Triphenyltetrazolium chloride (CAS 298-96-4, C19H15ClN4) is a heterocyclic organic compound with a molecular weight of 334.80 g/mol. This tetrazole derivative functions as a chromogenic redox indicator, particularly in biochemical assays. It is widely used as a substrate for dehydrogenase enzymes, where its reduction yields a red formazan precipitate, enabling the quantitative assessment of enzyme activity. It also serves as a vital building block in chemical synthesis.
- IUPAC
- TPTZ,TTZ,Tetrazolium Red
- Synonyms
- 298-96-4C19H15ClN4Tetrazolium RedTPTZTTZ
Biochemical Assays and Enzyme Activity Measurement
Utilised as a key substrate for detecting dehydrogenase activity in various biological samples. Reduction of 2,3,5-Triphenyltetrazolium chloride produces a distinct red formazan, facilitating the quantification of enzyme kinetics and metabolic activity.
Cell Viability and Cytotoxicity Testing
Serves as a indicator in cell-based assays to assess metabolic activity, correlating with cell viability. Changes in colour intensity can indicate the effects of compounds on cellular respiration and overall cell health.
Histochemistry and Staining
Employed as a staining agent in histochemical studies to visualise enzyme activity within tissue sections. Its ability to form a coloured precipitate allows for the localisation of specific enzymatic processes.
Chemical Synthesis Intermediate
Acts as a versatile building block in organic synthesis for the creation of more complex heterocyclic compounds. Its tetrazole ring structure offers unique reactivity for further chemical modifications.
| Molecular weight | 334.80 |
|---|---|
| Empirical formula | C19H15ClN4 |
| Assay | 98.0-102.0% |
| Grade | SAJ special grade |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Availability | available only in Japan |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 2,3,5-Triphenyltetrazolium chloride used for?
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2,3,5-Triphenyltetrazolium chloride is primarily used as a chromogenic substrate for detecting dehydrogenase activity, a colourimetric indicator in cell viability assays, and as a building block in chemical synthesis.
What are the CAS number and formula for 2,3,5-Triphenyltetrazolium chloride?
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The CAS number is 298-96-4 and the empirical formula is C19H15ClN4.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 2,3,5-Triphenyltetrazolium chloride in SAJ special grade, with an assay of 98.0-102.0%. This product is not represented as USP, BP, or EP grade.
How should 2,3,5-Triphenyltetrazolium chloride be handled safely?
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Handle with appropriate personal protective equipment, including a dust mask (type N95 or equivalent), eyeshields, and gloves. Avoid inhalation of dust and contact with skin and eyes, as it can cause skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335).
Is 2,3,5-Triphenyltetrazolium chloride available for export?
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2,3,5-Triphenyltetrazolium chloride is available only in Japan. Tech Serve Solutions can discuss export possibilities for other regions upon inquiry.
How can I request a quote or sample?
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Please contact our sales department directly through our website or by phone to request a quote or sample for 2,3,5-Triphenyltetrazolium chloride.
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