16β-Hydroxytestosterone
16β-Hydroxytestosterone (CAS: 17528-90-4), a steroid compound with the empirical formula C19H28O3 and a molecular weight of 304.42 g/mol, is a vital research chemical. It serves primarily as a standard in chromatographic analyses, specifically for the quantification of testosterone hydroxylases. This compound is integral to studies within cell biology, cell signaling, and drug metabolism research.
- IUPAC
- 16β,17β-Dihydroxyandrost-4-en-3-one,4-Androstene-16β,17β-diol-3-one
- Synonyms
- 16β,17β-Dihydroxyandrost-4-en-3-one4-Androstene-16β,17β-diol-3-one

Chromatographic Standard
Utilised as a reference standard in high-performance liquid chromatography (HPLC) and other chromatographic techniques for the assay of testosterone hydroxylase enzymes.
Metabolism Research
A key compound for investigating drug and xenobiotic metabolism, particularly pathways involving steroid transformations and interactions with multi-drug resistance mechanisms.
Cell Signaling Studies
Employed in research focused on cell signaling pathways and neurochemical processes where steroid hormones play a regulatory role.
| Molecular weight | 304.42 |
|---|---|
| Empirical formula | C19H28O3 |
| Storage temperature | 2-8°C |
| Application | 16β-Hydroxytestosterone can be used as a standard for chromatographic assays of testosterone hydroxylases. |
| Melting point | 184-186 |

Hazard statements
- H351Suspected of causing cancer
- H360May damage fertility or the unborn child
Precautionary statements
- P201Obtain special instructions before use
- P281Use personal protective equipment as required
- P308IF exposed or concerned
| Protective equipment | Eyeshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 61-40 |
| Safety statements (S) | 22-36/37/39 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 16β-Hydroxytestosterone used for?
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16β-Hydroxytestosterone is primarily used as a standard for chromatographic assays of testosterone hydroxylases and in research related to cell biology, cell signaling, drug metabolism, and neuroscience.
What are the CAS number and chemical formula for 16β-Hydroxytestosterone?
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The CAS number for 16β-Hydroxytestosterone is 17528-90-4, and its empirical formula is C19H28O3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 16β-Hydroxytestosterone as a research-grade chemical. The specific assay and purity are detailed on the product's Certificate of Analysis. TSS does not represent this product as USP, BP, EP, or other pharmacopoeial grades unless explicitly stated.
What are the safety considerations for handling 16β-Hydroxytestosterone?
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16β-Hydroxytestosterone is classified as Danger (GHS08) with hazards H351 (suspected of causing cancer) and H360 (may damage fertility or the unborn child). Appropriate personal protective equipment, including eyeshields, gloves, and respiratory protection (e.g., N100 respirator), is essential during handling.
How is 16β-Hydroxytestosterone stored and shipped?
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This compound should be stored at 2-8°C. Tech Serve Solutions offers reliable global export services for research chemicals, adhering to all relevant shipping regulations.
How can I request a quote or sample?
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To request a quote or a sample of 16β-Hydroxytestosterone, please contact our sales department via the website or direct email, providing your contact details and required quantity.
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