1,8-Diazafluoren-9-one
1,8-Diazafluoren-9-one (CAS: 54078-29-4) is a chemical compound with the molecular formula C11H6N2O and a molecular weight of 182.18 g/mol. It is recognised for its utility as an analytical reagent, particularly in forensic science for the detection of latent fingerprints. This compound reacts with amino acids to produce highly fluorescent derivatives, enabling sensitive visualisation.
- IUPAC
- Cyclopenta[1,2-b:4,3-b’]dipyridin-9-one
- Synonyms
- C11H6N2OCyclopenta[1,2-b:4,3-b’]dipyridin-9-one

Latent Fingerprint Detection
In forensic applications, 1,8-Diazafluoren-9-one is employed to visualise latent fingerprints on various surfaces, especially paper. It reacts with amino acids present in fingerprint residues, forming fluorescent products that aid in identification.
Amino Acid Analysis
The compound serves as a reagent for detecting and quantifying amino acids. Upon reaction, it generates highly fluorescent derivatives, allowing for sensitive analysis via fluorescence spectroscopy with excitation and emission maxima at approximately 470 nm and 570 nm, respectively.
Fluorescent Labelling
As a fluorescent label, 1,8-Diazafluoren-9-one can be used in biochemical and analytical contexts where the detection of amino acid-containing molecules is required. Its ability to form fluorescent adducts makes it valuable for sensitive detection methods.
| Molecular weight | 182.18 |
|---|---|
| Empirical formula | C11H6N2O |
| Solubility | acetic acid: soluble10 mg/mL, clear |
| Storage temperature | 2-8°C |
| Application | 1,8-Diazafluoren-9-one (DFO) is used in forensic science to detect latent fingerprints on paper and other materials. DFO forms highly fluorescent derivatives with amino acids (excitation ~470 nm; emission ~570 nm). |
| Melting point | 229-233 °C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 470 nm; λem 570 nm |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 1,8-Diazafluoren-9-one used for?
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1,8-Diazafluoren-9-one is primarily used in forensic science for the detection of latent fingerprints on paper and other materials. It also functions as a reagent for amino acid analysis due to its ability to form highly fluorescent derivatives with amino acids.
What are the CAS number and molecular formula for 1,8-Diazafluoren-9-one?
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The CAS number for 1,8-Diazafluoren-9-one is 54078-29-4, and its molecular formula is C11H6N2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 1,8-Diazafluoren-9-one for research and analytical purposes. The specific grade and purity are detailed in the product's specification sheet. TSS does not represent this product as USP, BP, or EP grade unless explicitly stated.
How should 1,8-Diazafluoren-9-one be handled and stored safely?
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Handling 1,8-Diazafluoren-9-one requires appropriate personal protective equipment, including eyeshields, gloves, and a P1 (EN143) or N95 respirator filter. It is classified with WGK Germany 3, indicating a significant hazard to water. Store the compound at 2-8°C.
How is 1,8-Diazafluoren-9-one packed, shipped, and exported?
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Tech Serve Solutions adheres to stringent international regulations for the packing, shipping, and export of chemicals, ensuring safe and compliant delivery of 1,8-Diazafluoren-9-one to customers worldwide.
How can I request a sample or a quote for 1,8-Diazafluoren-9-one?
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To request a sample or obtain a quote for 1,8-Diazafluoren-9-one, please contact our sales department through the website or by direct inquiry, providing your specific requirements.
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