Applications

4-Bromomethyl-6,7-dimethoxycoumarin was used as fluorescent labeling reagent for the reversed-phase HPLC separation and detection of carboxylic acids.^1,2 It was also used as derivatization reagent:
• for quantitation of clofibric, bezafibric and fenofibric acids in liver by liquid chromatography³
• in determination of the concentration of tripeptide glutathione by matrix-assisted laser desorption ionization time-of-flight mass spectrometry⁴
• for determination of carboxylic acids in chromatographic detection⁵