Applications

Trypan blue is a blue acid dye that contains two azo chromophores. It is a large, hydrophilic, tetrasulfonated dye. Trypan blue has been used as a vital stain as it is excluded by most living cells. It has been used to stain collagen and amyloid. It is also used in chlamydospore agar for fungi. When Trypan blue binds to proteins the resulting complex emits red fluorescence.