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3,3′,5,5′-Tetramethylbenzidine dihydrochloride

3,3′,5,5′-Tetramethylbenzidine dihydrochloride chemical structure
CAS Number64285-73-0
Formula5
Mol. Weight313.27 g/mol

What is 3,3′,5,5′-Tetramethylbenzidine dihydrochloride?

3,3′,5,5′-Tetramethylbenzidine dihydrochloride is a chemical compound with CAS number 64285-73-0 and molecular formula 5. It is used in various industrial and research applications.

Applications

3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

Specifications

Molecular Weight

313.27 g/mol

Frequently Asked Questions

Alternative Names & Synonyms

5′-Tetramethylbenzidine dihydrochloride,64285-73-0, C16H20N2 · 2HCl