Guanidine hydrochloride
Guanidine hydrochloride
What is Guanidine hydrochloride?
Guanidine hydrochloride is a chemical compound with CAS number 50-01-1 and molecular formula NH2C(=NH)NH2 · HCl. It is used in various industrial and research applications.
Applications
Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.
Specifications
NH2C(=NH)NH2 · HCl
95.53 g/mol
for molecular biology
≥99.0%
DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected
1.18 g/mL at 25 °C(lit.)
H2O: soluble6 M at 20 °C, clear, colorless
Safety Information
GHS07Warning
H302-H315-H319
P305 + P351 + P338
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
180-185 °C(lit.)
≤0.05% (as SO4)
sulfate (SO42-): ≤50 mg/kg
Al: ≤5 mg/kg
As: ≤0.1 mg/kg
Ba: ≤5 mg/kg
Bi: ≤5 mg/kg
Ca: ≤10 mg/kg
Cd: ≤5 mg/kg
Co: ≤5 mg/kg
Cr: ≤5 mg/kg
Cu: ≤5 mg/kg
Fe: ≤5 mg/kg
K: ≤50 mg/kg
Li: ≤5 mg/kg
Mg: ≤5 mg/kg
Mn: ≤5 mg/kg
Mo: ≤5 mg/kg
Na: ≤50 mg/kg
Ni: ≤5 mg/kg
Pb: ≤5 mg/kg
Sr: ≤5 mg/kg
Zn: ≤5 mg/kg
200-002-3
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