Isopropyl β-D-1-thiogalactopyranoside
CAS Number367-93-1
FormulaC9H18O5S
Mol. Weight238.30 g/mol
Isopropyl β-D-1-thiogalactopyranoside
What is Isopropyl β-D-1-thiogalactopyranoside?
Isopropyl β-D-1-thiogalactopyranoside is a chemical compound with CAS number 367-93-1 and molecular formula C9H18O5S. It is used in various industrial and research applications.
Applications
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Specifications
Molecular Weight
238.30 g/mol
Assay/Purity
≥99% (TLC)
Appearance
non-ionic
Impurities
10.0-22.0% Dioxane