4′,6-Diamidino-2-phenylindole dihydrochloride
4′,6-Diamidino-2-phenylindole dihydrochloride
What is 4′,6-Diamidino-2-phenylindole dihydrochloride?
4′,6-Diamidino-2-phenylindole dihydrochloride is a chemical compound with CAS number 28718-90-3. It is used in various industrial and research applications.
Applications
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Specifications
350.25 g/mol
≥98% (HPLC and TLC)
H2O: soluble20 mg/mL (Heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.)
PBS: insoluble