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Ethidium bromide

Ethidium bromide chemical structure
CAS Number1239-45-8
FormulaC21H20BrN3
Mol. Weight394.31 g/mol

What is Ethidium bromide?

Ethidium bromide is a chemical compound with CAS number 1239-45-8 and molecular formula C21H20BrN3. It is used in various industrial and research applications.

Applications

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10

Specifications

Molecular Weight

394.31 g/mol

Assay/Purity

~95% (HPLC)

Safety Information

GHS06GHS06
GHS08GHS08
Signal Word

Danger

Hazard Statements

H302-H330-H341

Precautionary Statements

P260-P281-P284-P310

Required PPE

Eyeshields, Faceshields, full-face particle respirator type N100 (US), Gloves, respirator cartridge type N100 (US), type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

Melting Point

260-262 °C (dec.)(lit.)

EC Number

214-984-6

WGK Germany

3

Frequently Asked Questions