Nitro Blue Tetrazolium

Nitro Blue Tetrazolium (CAS: 298-83-9) is a colourless to pale yellow crystalline solid, primarily utilised as a chromogenic substrate. It is employed in biochemical assays, particularly for the detection of alkaline phosphatase activity. This compound is indispensable in various diagnostic and research applications, enabling the visual identification of enzyme-linked reactions. Its reliable performance makes it a staple reagent for laboratories worldwide.

IUPAC

2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride,3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride],p-Nitro-Blue tetrazolium chloride,p-Nitrotetrazolium blue,NBT,Nitro BT,Nitrotetrazolium Blue chloride

Synonyms

p-Nitro-Blue tetrazoliump-Nitrotetrazolium blueNBTNitro BTNitrotetrazolium Blue chloride2,2′-bis(4-Nitrophenyl)-5,5′-diphenyl-3,3′-(3,3′-dimethoxy-4,4′-diphenylene)ditetrazolium chloride3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride]