Ethylenediaminetetraacetic acid tetrasodium salt dihydrate
Ethylenediaminetetraacetic acid tetrasodium salt dihydrate (CAS: 10378-23-1) is a versatile chelating agent with a molecular weight of 416.20 g/mol. It is known for its ability to bind metal ions, making it valuable in various laboratory and industrial applications. This compound is particularly effective in eliminating enzyme inhibition caused by trace heavy metals and inhibiting enzymes that require divalent cations as cofactors, ensuring accurate experimental results.
- IUPAC
- EDTA tetrasodium salt,Edathamil,Tetrasodium ethylenediaminetetraacetate dihydrate
- Synonyms
- EDTA tetrasodium saltEdathamilTetrasodium ethylenediaminetetraacetate dihydrate

Enzyme Activity Modulation
This tetrasodium salt is employed to counteract enzyme inhibition by trace heavy metals. It also serves to inhibit enzymes that depend on divalent cations as essential cofactors, thereby controlling their activity in biochemical assays.
Chelation of Metal Ions
As a powerful chelating agent, it sequesters various metal ions from solution. This property is crucial in applications requiring the removal or control of metal ion concentrations, such as in water treatment or preventing metal-catalyzed degradation.
Cell Biology and Culture
Its ability to chelate calcium ions makes it useful in cell dissociation protocols and for maintaining cell cultures by preventing calcium-dependent aggregation or signalling.
Laboratory Reagent
It functions as a general laboratory reagent in analytical chemistry and various research settings where metal ion control is necessary for precise outcomes.
| Molecular weight | 416.20 |
|---|---|
| Linear formula | (NaOOCCH2)2NCH2CH2N(CH2COONa)2 · 2H2O |
| Assay | 99.0-102.0% (titration) |
| Solubility | H2O: soluble, clear, colorless |
| Application | Used to eliminate enzyme inhibition by traces of heavy metals, and to inhibit enzymes that require divalent cations as cofactors. |


Hazard statements
- H302Harmful if swallowed
- H318Causes serious eye damage
Precautionary statements
- P280Wear protective gloves, clothing and eye/face protection
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 2 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-41 |
| Safety statements (S) | 26-39-46 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Ethylenediaminetetraacetic acid tetrasodium salt dihydrate used for?
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It is used to eliminate enzyme inhibition by traces of heavy metals and to inhibit enzymes that require divalent cations as cofactors. It also finds applications in cell biology and as a general laboratory reagent for metal ion control.
What is the CAS number and formula for this compound?
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The CAS number is 10378-23-1, and the linear formula is (NaOOCCH2)2NCH2CH2N(CH2COONa)2 · 2H2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Ethylenediaminetetraacetic acid tetrasodium salt dihydrate with an assay of 99.0-102.0% (titration). TSS does not represent this product as USP, BP, or EP grade unless explicitly stated.
What are the safety and handling precautions for this chemical?
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This compound is dangerous. It can cause serious eye damage (H318) and is harmful if swallowed (H302). Always wear eye protection, gloves, and a dust mask (type N95 US). In case of contact with eyes, rinse cautiously with water for several minutes and seek immediate medical attention.
How is this product packaged and shipped?
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Products are carefully packaged to ensure integrity during transit and are exported globally by Tech Serve Solutions, adhering to all relevant shipping regulations.
How can I request a sample or a quote for this product?
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Please use the 'Request a Quote' or 'Sample Request' form on our website, or contact our sales team directly with your inquiry.
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