Bicinchoninic acid disodium salt hydrate
Bicinchoninic acid disodium salt hydrate (CAS 979-88-4) is a vital reagent for protein determination, offered by TSS. With the empirical formula C20H10N2Na2O4 · xH2O and an anhydrous molecular weight of 388.28 g/mol, it is crucial for biochemical research and diagnostics. This hydrate form is primarily utilised in spectrophotometric methods for accurately quantifying protein concentrations in biological samples.
- IUPAC
- 2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt,4,4′-Dicarboxy-2,2′-biquinoline disodium salt,BCA,Disodium 2,2′-biquinoline-4,4′-dicarboxylate
- Synonyms
- 2,2′-Biquinoline-4,4′-dicarboxylic acid disodium salt4,4′-Dicarboxy-2,2′-biquinoline disodium saltBCADisodium 2,2′-biquinoline-4,4′-dicarboxylate
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Protein Concentration Determination
Bicinchoninic acid disodium salt hydrate is a key component in the BCA assay for determining the total protein concentration in solutions. It forms a stable, coloured complex with copper(I) ions produced during the reduction of Cu(II) by proteins in an alkaline medium.
Biochemical Research
This reagent is widely employed in molecular biology and proteomics laboratories for essential protein quantitation. Accurate protein measurement is fundamental for experimental reproducibility and data integrity across various research fields.
Quantitation Kits and Reagents
It serves as a critical reagent in commercially available protein quantitation kits. TSS supplies this high-purity compound for manufacturers developing or refining their assay kits for protein analysis.
| Molecular weight | 388.28 (anhydrous basis) |
|---|---|
| Empirical formula | C20H10N2Na2O4 · xH2O |
| Assay | ≥98% (HPLC) |
| Solubility | 0.5% sodium bicarbonate: soluble50 mg/mL |
| Storage temperature | 2-8°C |
| Application | For the determination of protein concentration. |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H334May cause allergy or asthma symptoms if inhaled
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
- P342
| Protective equipment | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/38 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Bicinchoninic acid disodium salt hydrate used for?
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Bicinchoninic acid disodium salt hydrate is primarily used for the determination of protein concentration in biological samples. It is a key reagent in the BCA assay.
What are the CAS number and formula for Bicinchoninic acid disodium salt hydrate?
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The CAS number for the anhydrous form is 979-88-4. The empirical formula is C20H10N2Na2O4 · xH2O.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Bicinchoninic acid disodium salt hydrate with an assay of ≥98% by HPLC. We do not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should Bicinchoninic acid disodium salt hydrate be handled safely?
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This compound is classified with Danger signal word and carries hazard statements H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H334 (May cause allergy or asthma symptoms or breathing difficulties if inhaled). Appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, face shields, and gloves, must be worn. Handle in a well-ventilated area or under a fume hood. If inhaled, seek immediate medical attention. Refer to the Safety Data Sheet for comprehensive handling information.
Is Bicinchoninic acid disodium salt hydrate a controlled substance?
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The provided data does not indicate that Bicinchoninic acid disodium salt hydrate is a controlled substance.
How is Bicinchoninic acid disodium salt hydrate packaged and shipped?
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Bicinchoninic acid disodium salt hydrate is carefully packaged to ensure stability and integrity during transit. TSS exports fine chemicals globally, adhering to all relevant shipping regulations for hazardous materials.
How can I request a sample or quote for Bicinchoninic acid disodium salt hydrate?
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To request a sample or a quotation, please contact our sales team via the website or call us directly. We will be pleased to assist you with your specific requirements.
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