Arsenazo III
Arsenazo III (CAS: 1668-00-4) is a chemical compound with a molecular weight of 776.37. This analytical reagent is primarily utilized for the spectrophotometric determination of calcium in biological samples, such as serum. It also finds application in assessing calcium transport in cellular studies and for the quantitative analysis of uranium and thorium. Supplied by Tech Serve Solutions, it is a valuable tool in laboratory settings.
- IUPAC
- 2,2′-(1,8-Dihydroxy-3,6-disulfonaphthylene-2,7-bisazo)bisbenzenearsonic acid,2,7-Bis(2-arsonophenylazo)chromotropic acid

Calcium Determination
Arsenazo III is employed for the spectrophotometric determination of calcium, particularly in serum samples. It aids in understanding calcium levels in biological and clinical research.
Cellular Calcium Transport Studies
This reagent has been used to evaluate calcium transport mechanisms in permeabilised cells, contributing to research in cell biology and physiology.
Uranium and Thorium Analysis
Arsenazo III serves as a spectrophotometric reagent for the quantitative determination of uranium and thorium, supporting analytical chemistry applications.
| Molecular weight | 776.37 |
|---|---|
| Linear formula | (HO)2C10H2(SO3H)2(N=NC6H4AsO3H2)2 |
| Form | powder |
| Solubility | ammonium hydroxide: soluble1 mg/mL |
| Impurities tested | <0.1% Ca |
| Application | Arsenazo III is used in the determination of calcium in serum.1,2 It has been used to evaluate calcium transport in permeabilized cells.3 Arsenazo III has also been used for the spectrophotometric determination of uranium and thorium.4,5 |
| Melting point | >320 °C(lit.) |


Hazard statements
- H301Toxic if swallowed
- H410Very toxic to aquatic life with long-lasting effects
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P273Avoid release to the environment
- P301IF SWALLOWED
- P311Call a POISON CENTER or doctor
- P501Dispose of contents and container per local regulations
| Protective equipment | Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges |
|---|---|
| Transport (UN / ADR) | UN 3465 6.1 / PGII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T,N |
| Risk statements (R) | 23/25-50/53 |
| Safety statements (S) | 20/21-28-45-60-61 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Absorption | λmax 530 nm |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Arsenazo III used for?
+
Arsenazo III is used for the spectrophotometric determination of calcium in serum, evaluating calcium transport in permeabilised cells, and for the quantitative analysis of uranium and thorium.
What is the CAS number and formula for Arsenazo III?
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The CAS number for Arsenazo III is 1668-00-4. Its linear formula is (HO)₂C₁₀H₂(SO₃H)₂(N=NC₆H₄AsO₃H₂)₂.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Arsenazo III as a powder with an impurity level of <0.1% Ca. TSS does not represent this product as USP, BP, or EP grade.
How should Arsenazo III be handled safely?
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Arsenazo III is classified as toxic if swallowed or inhaled and very toxic to aquatic life with long-lasting effects. Handle with appropriate personal protective equipment, including eyeshields, faceshields, gloves, and a P2 respirator. Ensure adequate ventilation and avoid release into the environment.
How is Arsenazo III packed, shipped, and exported?
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Arsenazo III is packed and shipped according to relevant regulations for hazardous materials, including UN 3465 classification. Tech Serve Solutions offers global export services.
How can I request a quote or sample for Arsenazo III?
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To request a quote or sample of Arsenazo III, please contact the Tech Serve Solutions sales team through our website or by phone.
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