Xylene Cyanol FF
Xylene Cyanol FF (CAS: 2650-17-1), with the molecular formula C25H27N2NaO6S2 and a molecular weight of 538.61 g/mol, is a vital laboratory reagent. This blue powder functions effectively as a histological stain and a tracking dye in molecular biology applications, particularly in DNA sequencing and electrophoresis, aiding in visualising sample migration. Its distinct colour index (42135) contributes to its utility in these scientific disciplines.
- IUPAC
- Acid blue 147,Cyanol FF,XC,Xylene Cyanole FF
- Synonyms
- Acid blue 147Cyanol FFXCXylene Cyanole FF

Histological Staining
Xylene Cyanol FF serves as a reliable stain in histology. It is used to visualise cellular structures and tissues, contributing to accurate microscopic examination and diagnosis in biological research and medical laboratories.
DNA/RNA Electrophoresis Tracking
This compound acts as an effective tracking dye in gel electrophoresis for DNA and RNA. Its migration through the gel allows researchers to monitor the progress of the separation and estimate the size of nucleic acid fragments.
Molecular Biology Reagent
As a core bioreagent in molecular biology, Xylene Cyanol FF is employed in various experimental setups. Its properties make it suitable for applications demanding visual confirmation of reagent movement or sample separation.
| Molecular weight | 538.61 |
|---|---|
| Empirical formula | C25H27N2NaO6S2 |
| Grade | for molecular biology |
| Form | powder |
| Solubility | H2O: complete1 mg/mL, clear, blue |
| Storage temperature | room temp |
| Application | Xylene Cyanol FF is used as histological stain and as tracker dye in DNA sequencing and electrophoresis.1,2 |
| Melting point | 295 °C (dec.)(lit.) |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 42135 |
|---|---|
| Product Line | BioReagent |
| Absorption | λmax 615 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Xylene Cyanol FF used for?
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Xylene Cyanol FF is primarily used as a histological stain and as a tracking dye in DNA sequencing and electrophoresis.
What are the CAS number and molecular formula for Xylene Cyanol FF?
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The CAS number for Xylene Cyanol FF is 2650-17-1, and its molecular formula is C25H27N2NaO6S2.
What grade and purity does Tech Serve Solutions supply for Xylene Cyanol FF?
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Tech Serve Solutions supplies Xylene Cyanol FF in a 'for molecular biology' grade. TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade.
What are the safety considerations when handling Xylene Cyanol FF?
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Xylene Cyanol FF is classified with a Warning signal word, with hazard statements including H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation). Appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, and gloves, should be used. Handle in a well-ventilated area.
How is Xylene Cyanol FF packed and shipped?
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Tech Serve Solutions packs and exports Xylene Cyanol FF, typically supplied as a powder, suitable for research and laboratory use. Specific packaging details are available upon request.
How can I request a quote or sample for Xylene Cyanol FF?
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To request a quote or a sample of Xylene Cyanol FF, please contact our sales team through the website or by calling us directly. We are happy to assist with your specific requirements.
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