Rhodamine 123
Rhodamine 123 (CAS: 62669-70-9; Formula: C21H17ClN2O3; MW: 380.82) is a lipophilic fluorescent dye with significant applications in biological research. This membrane-permeant tracer is widely utilised for visualising cellular structures and dynamics, particularly the mitochondria, due to its selective accumulation within these organelles. Rhodamine 123 serves as a valuable tool in cell biology for assessing mitochondrial membrane potential and cellular viability.
- IUPAC
- 2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester
Mitochondrial Staining
Rhodamine 123 is a vital stain for visualising and quantifying mitochondrial membrane potential. Its lipophilic nature allows it to accumulate in the mitochondria of living cells, making it a key reagent for studying mitochondrial function and health.
Cell Viability Assays
The accumulation of Rhodamine 123 within mitochondria is directly related to the membrane potential, which in turn is linked to cellular viability. It is therefore employed in assays to assess the impact of various treatments on cell health and survival.
Flow Cytometry
As a fluorescent probe, Rhodamine 123 is useful in flow cytometry for discriminating between viable and non-viable cells, and for analysing mitochondrial function in cell populations following drug treatment or experimental manipulation.
Fluorescence Microscopy
In fluorescence microscopy, Rhodamine 123 enables high-resolution imaging of cellular structures, particularly the intricate network of mitochondria. Its bright fluorescence allows for clear visualisation in fixed and live-cell imaging experiments.
| Molecular weight | 380.82 |
|---|---|
| Linear formula | C21H17ClN2O3 |
| Grade | for fluorescence |
| Solubility | ethanol: soluble1 mg/mL |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Rhodamine 123 used for?
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Rhodamine 123 is primarily used as a lipophilic fluorescent dye for visualising cellular structures, especially mitochondria. It is valuable for assessing mitochondrial membrane potential and cellular viability in biological research and cell biology applications.
What are the CAS number and chemical formula for Rhodamine 123?
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The CAS number for Rhodamine 123 is 62669-70-9, and its chemical formula is C21H17ClN2O3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Rhodamine 123 in a "for fluorescence" grade. We do not represent this product as USP, BP, EP or other pharmacopoeial grades.
What safety precautions should be taken when handling Rhodamine 123?
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When handling Rhodamine 123, it is essential to wear appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter should be used if airborne exposure is possible. Always consult the Safety Data Sheet (SDS) before use.
How is Rhodamine 123 packed and shipped?
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Tech Serve Solutions packs and ships chemicals with care, adhering to all relevant international regulations for hazardous materials. Specific packaging details depend on the quantity ordered and the destination country's import laws.
How can I request a quote or sample for Rhodamine 123?
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To request a quote or a sample of Rhodamine 123, please contact our sales team via the website or by phone. Please include the product name, CAS number, and desired quantity for quotes, or specify 'sample' for sample requests.
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