Prussian blue soluble

CAS 12240-15-2C6Fe2KN6 · xH2OCell Biology

Prussian blue soluble (CAS: 12240-15-2), with the empirical formula C6Fe2KN6 · xH2O, is an inorganic pigment rather than a dye. It is crucial for demonstrating non-hemoglobin cytoplasmic iron and hemosiderin in histological and cell biology applications. It is also employed in combination with iron particles for tracking transplanted cells like mesenchymal stem cells and differentiating cell populations based on division rates.

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Prussian blue soluble (C6Fe2KN6 · xH2O) — chemical structure, CAS 12240-15-2; Life Science, fine chemical supplied by Tech Serve Solutions

Cell BiologyHematology and HistologyStains & DyesA to ZStains and Dyes

01 /Applications

Histological Staining

Prussian blue is used for the in situ demonstration of non-hemoglobin, cytoplasmic iron and hemosiderin. It follows established procedures by Sundberg and Broman, and Highman.

Cell Tracking

In conjunction with iron oxide nanoparticles, Prussian blue can be utilised for tracking transplanted cells, such as mesenchymal stem cells, within biological systems.

Cell Proliferation Studies

This compound can differentiate between slow and fast cycling cells by monitoring the dilution of iron oxide particle labelling through cell division.

02 /Properties

Molecular weight	306.89 (anhydrous basis)
Empirical formula	C6Fe2KN6 · xH2O
Application	Prussian blue is not a dye but an inorganic pigment containing ferric ferrocyanide. Procedures have been published1 for the demonstration of non-hemoglobin, cytoplasmic iron, according to Sundberg and Broman,2 and for the demonstration of hemosiderin according to Highman3 and from Lille and Fullmer4 based the work originally done by Perle in 1867.5 Prussian Blue is formed in situ in both of these methods. In combination with iron particles such as superparamagnetic iron oxide nanoparticles, Prussian blue may be used for tracking of transplanted cells such as mesenchymal stem cells.6,7 Prussian blue may also be used to differentiate slow versus fast cycling cells by methods that monitor iron oxide particle labeling dilution thru cell division.8

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

12240-15-2

MDL number

MFCD00135664

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is Prussian blue soluble used for?

Prussian blue soluble (CAS: 12240-15-2) is used for demonstrating non-hemoglobin, cytoplasmic iron and hemosiderin in cell biology and histology. It is also used with iron particles for tracking transplanted cells and differentiating cell cycling rates.

What is the CAS number and formula for Prussian blue soluble?

The CAS number for Prussian blue soluble is 12240-15-2, and its empirical formula is C6Fe2KN6 · xH2O.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies Prussian blue soluble, CAS 12240-15-2, with a molecular weight of 306.89 (anhydrous basis). We do not represent this product as USP, BP, EP, or any other pharmacopoeia standard.

What are the safety and handling requirements for Prussian blue soluble?

When handling Prussian blue soluble (CAS: 12240-15-2), appropriate personal protective equipment including eyeshields, gloves, and a P1 (EN143) or N95 respirator filter is recommended. The WGK Germany classification is 3.