Phenol – chloroform – isoamyl alcohol mixture
Phenol–chloroform–isoamyl alcohol mixture (CAS 136112-00-0) is a specialized reagent supplied by Tech Serve Solutions. This three-component solution is commonly utilised in molecular biology for DNA and RNA purification procedures. Its composition is designed to facilitate efficient cell lysis and denaturation of proteins, enabling the isolation of nucleic acids. Available as a BioUltra grade product, it meets stringent requirements for sensitive biological applications.
DNA and RNA Purification
This mixture is a key component in protocols for isolating DNA and RNA from biological samples. It aids in the denaturation of proteins and separation of nucleic acids from other cellular components.
Nucleic Acid Isolation
Utilised in various DNA and RNA extraction methods, particularly those requiring phenol-based separation for high purity nucleic acids.
Molecular Biology Reagent
As a BioUltra grade product, it is suitable for demanding molecular biology applications where reagent purity is critical for reliable experimental outcomes.
| Grade | for molecular biology |
|---|---|
| Density | 1.12 g/mL at 20 °C |
| pH | 4-5(H2O-phase, after extraction with H2O, 1:1) |
| Storage temperature | 2-8°C |
| Impurities tested | DNases, none detectedRNases, none detectedphosphatases, none detectedproteases, none detected |
Hazard statements
- H301Toxic if swallowed
- H312Harmful in contact with skin
- H314Causes severe skin burns and eye damage
- H336May cause drowsiness or dizziness
- H341Suspected of causing genetic defects
- H351Suspected of causing cancer
- H361D
- H373May cause damage to organs through prolonged exposure
- H411Toxic to aquatic life with long-lasting effects
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P273Avoid release to the environment
- P280Wear protective gloves, clothing and eye/face protection
- P301IF SWALLOWED
- P305IF IN EYES
- P310Immediately call a POISON CENTER or doctor
| Protective equipment | Faceshields, full-face respirator (US), Gloves, Goggles, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter |
|---|---|
| Transport (UN / ADR) | UN 2810 6.1 / PGII |
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | T |
| Risk statements (R) | 23/24/25-34-40-48/20/21/22-68 |
| Safety statements (S) | 26-36/37/39-45 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioUltra |
|---|---|
| Uv Absorption | λ: 330 nm Amax: 0.2λ: 405 nm Amax: 0.1λ: 510 nm Amax: 0.1 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Phenol–chloroform–isoamyl alcohol mixture used for?
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Phenol–chloroform–isoamyl alcohol mixture is primarily used for DNA and RNA purification in molecular biology applications. It facilitates cell lysis and denaturation of proteins for efficient nucleic acid isolation.
What is the CAS number and formula for Phenol–chloroform–isoamyl alcohol mixture?
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The CAS number is 136112-00-0. The chemical formula is not explicitly provided as it is a mixture.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product as a BioUltra grade, specifically intended for molecular biology applications. TSS does not represent this product as USP, BP, EP, or pharmacopoeia grade.
How should Phenol–chloroform–isoamyl alcohol mixture be handled safely?
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This mixture is dangerous and corrosive. Handle with extreme caution using appropriate personal protective equipment, including a full-face respirator, gloves, and goggles. Avoid inhalation, skin contact, and eye contact. Refer to the safety data sheet for detailed handling and emergency procedures.
Is this product packed and shipped globally?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, pharmaceutical intermediates, and laboratory reagents, including this mixture.
How can I request a quote or sample?
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To request a quote or a sample, please contact our sales team through the website or by calling our customer service line.
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