N,N,N′,N′-Tetramethylbenzidine
N,N,N′,N′-Tetramethylbenzidine (CAS 366-29-0) is a key chromogenic substrate with the molecular formula [-C<sub>6</sub>H<sub>4</sub>-4-N(CH<sub>3</sub>)<sub>2</sub>]<sub>2</sub> and a molecular weight of 240.34 g/mol. Supplied with an assay of ≥95% (HPLC), it is primarily utilised in biochemical assays and diagnostic applications, particularly as a sensitive substrate for peroxidase enzymes in ELISA and other detection methods. This compound facilitates clear colourimetric results.
- Synonyms
- N′-Tetramethylbenzidine366-29-0[-C6H4-4-N(CH3)2]2
Enzyme-Linked Immunosorbent Assay (ELISA) Substrate
N,N,N′,N′-Tetramethylbenzidine serves as an excellent chromogenic substrate for peroxidase enzymes in ELISA techniques. Its high sensitivity allows for the detection of even low concentrations of analytes, producing a distinct colour change upon enzymatic reaction.
Peroxidase Detection
This compound is widely used as a detection substrate for various peroxidases, including horseradish peroxidase (HRP). It is a preferred substrate due to its low background and high signal-to-noise ratio in biochemical and diagnostic assays.
Chromogenic Reagent
As a chromogenic reagent, N,N,N′,N′-Tetramethylbenzidine reacts with oxidising agents in the presence of peroxidase to produce a coloured product. This property makes it invaluable for visualising and quantifying biological processes.
| Molecular weight | 240.34 |
|---|---|
| Linear formula | [-C6H4-4-N(CH3)2]2 |
| Assay | ≥95% (HPLC) |
| Melting point | 193-195 °C(lit.) |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is N,N,N′,N′-Tetramethylbenzidine used for?
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N,N,N′,N′-Tetramethylbenzidine is primarily used as a sensitive chromogenic substrate for peroxidase enzymes, particularly in Enzyme-Linked Immunosorbent Assays (ELISA) and other biochemical detection methods.
What are the CAS number and formula for N,N,N′,N′-Tetramethylbenzidine?
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The CAS number for N,N,N′,N′-Tetramethylbenzidine is 366-29-0, and its linear formula is [-C<sub>6</sub>H<sub>4</sub>-4-N(CH<sub>3</sub>)<sub>2</sub>]<sub>2</sub>.
What grade and purity does Tech Serve Solutions supply for N,N,N′,N′-Tetramethylbenzidine?
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Tech Serve Solutions supplies N,N,N′,N′-Tetramethylbenzidine with an assay of ≥95% (HPLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeia grade.
What are the safety precautions for handling N,N,N′,N′-Tetramethylbenzidine?
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N,N,N′,N′-Tetramethylbenzidine is classified with Warning signal word and hazards H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation). Appropriate personal protective equipment, including a dust mask type N95 (US), eyeshields, and gloves, should be used. Ensure adequate ventilation and avoid inhaling dust.
How is N,N,N′,N′-Tetramethylbenzidine packed and exported by TSS?
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As a specialist global supplier and exporter, Tech Serve Solutions ensures appropriate packaging for N,N,N′,N′-Tetramethylbenzidine for safe handling, storage, and international shipment according to regulatory requirements.
How can I request a quote or sample for N,N,N′,N′-Tetramethylbenzidine?
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To request a quote or a sample of N,N,N′,N′-Tetramethylbenzidine, please contact our sales team through the website or by phone, providing your specific requirements.
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