L-Arginine p-nitroanilide dihydrochloride
L-Arginine p-nitroanilide dihydrochloride (CAS: 40127-11-5) is a valuable biochemical reagent with the molecular formula C12H18N6O3 · 2HCl and a molecular weight of 367.23 g/mol. This compound serves as a chromogenic substrate, primarily utilised in enzymatic assays. Its predictable reaction characteristics make it an important tool in biochemical research and diagnostic applications for detecting specific enzyme activities.

Enzyme Substrate in Biochemical Assays
L-Arginine p-nitroanilide dihydrochloride functions as a chromogenic substrate for the quantitative determination of enzyme activity. Upon enzymatic cleavage, it releases a coloured product, allowing for spectrophotometric measurement.
Research in Proteolytic Enzyme Activity
This compound is employed in laboratories to study and characterise proteases and other enzymes that specifically hydrolyse arginine-containing peptide bonds.
Diagnostic Reagent Development
Its utility extends to the development and validation of diagnostic kits and assays that rely on detecting enzymatic reactions through colourimetric changes.
| Molecular weight | 367.23 |
|---|---|
| Empirical formula | C12H18N6O3 · 2HCl |
| Storage temperature | 2-8°C |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is L-Arginine p-nitroanilide dihydrochloride used for?
+
L-Arginine p-nitroanilide dihydrochloride is used as a chromogenic substrate in biochemical assays, particularly for the determination of enzyme activity, such as proteases. It facilitates colourimetric detection upon enzymatic cleavage.
What are the CAS number and formula for L-Arginine p-nitroanilide dihydrochloride?
+
The CAS number is 40127-11-5, and the empirical formula is C12H18N6O3 · 2HCl.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this product for laboratory and research purposes. The specific assay and purity are detailed on the product's Certificate of Analysis. We do not represent this product as USP, BP, or EP grade unless explicitly stated.
How should L-Arginine p-nitroanilide dihydrochloride be handled and stored?
+
Handle with appropriate personal protective equipment, including eyeshields and gloves. A P1 (EN143) or N95 respirator filter is recommended. Store at 2-8°C as indicated by the supplier.
Is this chemical a controlled substance?
+
Based on the provided data, there is no indication that L-Arginine p-nitroanilide dihydrochloride is a controlled substance.
How can I request a sample or quote for L-Arginine p-nitroanilide dihydrochloride?
+
Please contact our sales department via the website or direct email to request a sample or a quotation. We export globally.
What is the molecular weight of L-Arginine p-nitroanilide dihydrochloride?
+
The molecular weight is 367.23 g/mol.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents

(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more

3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis

3-Methyl-1,5-pentanediol
Chemical Synthesis

3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis