Janus Green B
Janus Green B (CAS: 2869-83-2), with the molecular formula C30H31ClN6 and a molecular weight of 511.06 g/mol, is a distinctive phenazinium dye. It is recognised for its ability to selectively stain mitochondria and certain other cellular components, making it a valuable tool in biological and histological studies. This dye is essential for visualising specific cellular structures in various laboratory applications.
- IUPAC
- 3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chloride,Diazin Green S,Union Green B
- Synonyms
- 3-Diethylamino-7-(4-dimethylaminophenylazo)-5-phenylphenazinium chlorideDiazin Green SUnion Green B

Vital Staining of Mitochondria
Janus Green B is primarily used as a vital stain to specifically identify and visualise mitochondria within living cells. Its selective uptake by these organelles allows for detailed microscopic examination of mitochondrial morphology and distribution without immediate cell death.
Histological Staining
In histology, Janus Green B serves as a differential stain for various tissues. It aids in the identification of specific cell types and structures, particularly those with high mitochondrial content, contributing to accurate pathological and biological analysis.
Cell Biology Research
Researchers in cell biology utilise Janus Green B for studying cellular processes, viability, and the functional state of mitochondria. Its application helps in understanding cell metabolism and the impact of various treatments on cellular organelles.
| Molecular weight | 511.06 |
|---|---|
| Empirical formula | C30H31ClN6 |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 11050 |
|---|---|
| Absorption | λmax 395 nmλmax 660 nm (2nd) |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Janus Green B used for?
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Janus Green B is used as a vital stain for mitochondria and in histological applications for differential staining of cells and tissues. It is particularly valuable in cell biology research for visualising cellular structures.
What is the CAS number and molecular formula for Janus Green B?
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The CAS number for Janus Green B is 2869-83-2, and its molecular formula is C30H31ClN6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Janus Green B based on available specifications. Please refer to the product's Certificate of Analysis for precise details on assay and purity. TSS does not represent this product as USP, BP, or EP grade.
What are the safety and handling precautions for Janus Green B?
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Janus Green B requires careful handling. Recommended personal protective equipment includes eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is advised. The substance has a WGK Germany rating of 3, indicating a high hazard level.
How is Janus Green B packed, shipped, and exported?
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Tech Serve Solutions ensures that Janus Green B is appropriately packed and shipped in compliance with all relevant national and international regulations for chemical export. Specific packaging details are determined by order size and destination requirements.
How can I request a sample or quote for Janus Green B?
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To request a sample or a quote for Janus Green B, please contact our sales department through the website or by calling our listed phone number. Please provide your specific requirements and delivery details.
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