Isopropyl β-D-1-thiogalactopyranoside
Isopropyl β-D-1-thiogalactopyranoside (CAS: 367-93-1, C9H18O5S) is a non-ionic biochemical reagent with a molecular weight of 238.30 g/mol. Primarily utilised in molecular biology, IPTG is essential for inducing gene expression in cloning and protein expression studies. It functions by mimicking the natural inducer of the lac operon, isopropyl β-D-galactopyranoside, thereby facilitating the controlled expression of target genes in bacterial systems.
- IUPAC
- IPTG,Isopropyl β-D-thiogalactoside
- Synonyms
- Isopropyl β-D-thiogalactoside
Blue-White Screening
IPTG is a critical component in the blue-white screening method for identifying recombinant bacterial colonies. It induces the expression of the lacZ gene, leading to the production of functional β-galactosidase.
Protein Expression Induction
Used in conjunction with an appropriate reporter gene system, IPTG effectively induces the expression of recombinant proteins in bacterial hosts like E. coli, enabling researchers to produce proteins of interest.
Gene Expression Studies
IPTG serves as a molecular tool to control and study gene regulation, particularly within systems employing the lac operon, allowing for precise manipulation of gene transcription.
| Molecular weight | 238.30 |
|---|---|
| Empirical formula | C9H18O5S |
| Assay | ≥99% (TLC) |
| Storage temperature | 2-8°C |
| Impurities tested | 10.0-22.0% Dioxane |
| Application | IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. |
Precautionary statements
- P281Use personal protective equipment as required
- P305IF IN EYES
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Description | non-ionic |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Isopropyl β-D-1-thiogalactopyranoside used for?
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Isopropyl β-D-1-thiogalactopyranoside (IPTG) is primarily used in molecular biology for the induction of gene expression, particularly in blue-white screening for recombinant bacterial colonies and for inducing the expression of target proteins in systems utilizing the lac operon.
What are the CAS number and formula for Isopropyl β-D-1-thiogalactopyranoside?
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The CAS number is 367-93-1, and the empirical formula is C9H18O5S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Isopropyl β-D-1-thiogalactopyranoside with an assay of ≥99% (TLC). This product is not represented as USP, BP, EP, or any pharmacopoeia grade. It contains 10.0-22.0% Dioxane as an impurity. Please refer to the product specifications for full details.
How should Isopropyl β-D-1-thiogalactopyranoside be handled and stored?
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Handle with appropriate personal protective equipment including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter may be necessary. Store at 2-8°C to maintain stability. Observe precautionary statements P281 and P305 + P351 + P338.
Is Isopropyl β-D-1-thiogalactopyranoside a controlled substance?
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The provided data does not indicate that Isopropyl β-D-1-thiogalactopyranoside is a controlled substance.
How can I request a quote or sample for Isopropyl β-D-1-thiogalactopyranoside?
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Please contact the Tech Serve Solutions sales team directly through our website or via your usual contact channels to request a quote or sample.
Is this product available for export?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including Isopropyl β-D-1-thiogalactopyranoside.
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