Indigo
Indigo (CAS: 482-89-3, C16H10N2O2) is a vibrant organic dye known for its historical significance and versatile applications. With a molecular weight of 262.26 g/mol, this compound exhibits a characteristic absorption maximum at 602 nm. Indigo is widely utilised as a histological stain, a redox indicator in analytical chemistry, and as a key component in the production of dyes for textiles, particularly denim.
- IUPAC
- Indigo blue,Indigotin
- Synonyms
- Indigo blueIndigotin

Histological Staining
Indigo serves as an effective stain in histology, aiding in the microscopic examination of tissue samples. Its ability to colour specific cellular structures makes it valuable for diagnostic and research purposes in biological sciences.
Redox Indicator
In analytical chemistry, Indigo functions as a redox indicator. It can undergo reversible oxidation and reduction, making it useful for monitoring the progress of titrations and other redox reactions.
Dye Production
Historically and currently, Indigo is a crucial dye, most famously for colouring denim. It provides a distinct blue hue and is a staple in the textile industry for apparel and other fabric applications.
Bioactive Research
As a bioactive small molecule, Indigo is explored in various biological research contexts. Its interactions within cellular systems are of interest for understanding biochemical pathways.
| Molecular weight | 262.26 |
|---|---|
| Empirical formula | C16H10N2O2 |
| Composition | Dye content, 95% |
| Melting point | >300 °C(lit.) |

Hazard statements
- H373May cause damage to organs through prolonged exposure
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Flash point | >220 °C / >428 °F |
| Water hazard class (WGK, DE) | 1 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 48/20/21/22 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Colour Index Number | 73000 |
|---|---|
| Gene Information | human ... EPHX2(2053)mouse ... Ephx2(13850) |
| Absorption | λmax 602 nm |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Indigo used for?
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Indigo is primarily used as a histological stain, a redox indicator in analytical chemistry, and a dye for textiles, most notably denim. It is also investigated as a bioactive small molecule in biological research.
What are the CAS number and formula for Indigo?
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The CAS number for Indigo is 482-89-3, and its molecular formula is C16H10N2O2.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Indigo with a dye content of 95%. We do not represent this product as USP, BP, EP, or pharmacopoeia grade unless explicitly stated in the product specifications.
How should Indigo be handled safely?
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Indigo is classified as a substance that may cause damage to organs through prolonged or repeated exposure (H373). When handling, it is recommended to use a dust mask type N95, eyeshields, and gloves. Ensure adequate ventilation.
Is Indigo regulated or controlled?
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The provided data does not indicate that Indigo is a controlled substance.
How is Indigo packed and shipped by TSS?
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Tech Serve Solutions exports fine chemicals globally. Specific packing and shipping details for Indigo will be provided upon request to ensure compliance with international regulations.
How can I request a sample or quote for Indigo?
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To request a sample or a quote for Indigo, please contact our sales team through the TSS website or by direct inquiry, providing your specific requirements.
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