Biotin (5-fluorescein) conjugate
Biotin (5-fluorescein) conjugate (CAS: 957494-27-8) is a versatile biochemical reagent with the molecular formula C33H32N4O8S. This compound facilitates the labeling of avidin/streptavidin conjugated molecules, such as antibodies, with the 5-fluorescein fluorophore. It is particularly useful in fluorescence-based applications, offering a reliable method for introducing fluorescent tags into biological systems for detection and analysis. TSS supplies this conjugate for research and development purposes.
- IUPAC
- N-(Biotinylamidoethyl)-fluorescein-5-carboxamide,N-(Fluorescein-5-carbamidoethyl)biotinamide
Fluorescent Labeling of Biotinylated Molecules
This conjugate serves as a reagent for labeling avidin or streptavidin, which are commonly conjugated to antibodies. The 5-fluorescein moiety provides fluorescence for detection and imaging applications.
Biochemical Assays and Research
Utilised in various biochemical and immunological assays where fluorescent detection is required. Its ability to bind to avidin/streptavidin makes it valuable for probing biological interactions and localisations.
Fluorescence Microscopy
Applicable in fluorescence microscopy techniques for visualising cellular structures or molecules tagged with avidin/streptavidin, offering a specific and sensitive detection method.
| Molecular weight | 644.69 |
|---|---|
| Empirical formula | C33H32N4O8S |
| Assay | ≥90% (HPCE) |
| Solubility | DMSO: soluble |
| Application | Biotin (5-fluorescein) conjugate is a reagent that may be used in situations similar to fluorescein isothiocyanate staining to label avidin/streptavidin conjugated molecules such as antibodies with the fluorophore 5-fluorescein. |
| Suitability | suitable for fluorescence |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Product Line | BioReagent |
|---|---|
| Fluorescence | λex 490 nm; λem 524 nm in 0.1 M Tris pH 8.0 |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is Biotin (5-fluorescein) conjugate used for?
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Biotin (5-fluorescein) conjugate is primarily used to label avidin/streptavidin conjugated molecules, such as antibodies, with the 5-fluorescein fluorophore for fluorescence-based detection and analysis in various biochemical and research applications.
What are the CAS number and formula for Biotin (5-fluorescein) conjugate?
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The CAS number is 957494-27-8 and the empirical formula is C33H32N4O8S.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies Biotin (5-fluorescein) conjugate with an assay of ≥90% (HPCE), suitable for fluorescence. We do not represent this product as USP, BP, or EP grade.
How should Biotin (5-fluorescein) conjugate be handled?
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Handling requires appropriate personal protective equipment, including eyeshields, gloves, and a type N95 (US) or type P1 (EN143) respirator filter. Refer to the Safety Data Sheet for comprehensive handling instructions.
Is this product suitable for fluorescence applications?
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Yes, this product is explicitly stated as 'suitable for fluorescence' and features fluorescence emission and excitation wavelengths (λex 490 nm; λem 524 nm).
How is this product typically exported and shipped?
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Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals. Specific shipping and export details can be provided upon request.
How can I request a quote or sample?
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Please contact our sales team through the website or via phone to request a quotation or a sample of Biotin (5-fluorescein) conjugate.
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