ADA
ADA (N-(2-Acetamido)iminodiacetic acid), CAS 26239-55-4, with a molecular weight of 190.15 g/mol, is a dicarboxylic acid derivative. This compound functions as a biological buffer component, particularly when formulated with sodium hydroxide and sodium chloride to achieve specific pH ranges. It is also utilised in the preparation of immobilised pH gradients, highlighting its utility in specialised biochemical and separation techniques.
- IUPAC
- N-(2-Acetamido)iminodiacetic acid,N-(Carbamoylmethyl)iminodiacetic acid
- Synonyms
- N-(2-Acetamido)iminodiacetic acidN-(Carbamoylmethyl)iminodiacetic acid

Biological Buffering Agent
ADA is employed as a component in biological buffers, especially when combined with sodium hydroxide and sodium chloride. This formulation allows for precise pH control, with a useful range typically between pH 6.4 and 7.4.
Immobilised pH Gradient (IPG) Preparation
The compound is utilised in the preparation of immobilised pH gradients (IPGs). IPGs are critical for high-resolution two-dimensional gel electrophoresis, enabling the separation of complex protein mixtures.
Chemical Synthesis
As an unnatural amino acid derivative, ADA can serve as a building block or intermediate in complex organic synthesis pathways, contributing to the creation of novel compounds.
| Molecular weight | 190.15 |
|---|---|
| Linear formula | H2NCOCH2N(CH2CO2H)2 |
| Assay | ≥98% (titration) |
| Solubility | 1 M NaOH: soluble0.05 M, clear, colorless |
| Impurities tested | ≤0.005% Phosphorus (P)≤0.1% Insoluble matter |
| Application | Biological buffer component with sodium hydroxide and sodium chloride (pH 5.67-7.57, useful pH range 6.4-7.4).1 Used to prepare immobilized pH gradients.2 |
| Melting point | 219 °C (dec.)(lit.) |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Cation traces | Al: ≤0.0005%Ca: ≤0.0005%Cu: ≤0.0005%Fe: ≤0.0005%K: ≤0.005%Mg: ≤0.0005%NH4+: ≤0.05%Pb: ≤0.001%Zn: ≤0.0005% |
| Anion traces | chloride (Cl-): ≤0.05%sulfate (SO42-): ≤0.05% |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Useful Ph Range | 6.0 - 7.2 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is ADA used for?
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ADA is used as a biological buffer component and in the preparation of immobilised pH gradients. It also finds application in chemical synthesis and peptide chemistry.
What is the CAS number and formula for ADA?
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The CAS number for ADA is 26239-55-4. Its linear formula is H₂NCOCH₂N(CH₂CO₂H)₂.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies ADA with a purity of ≥98% (titration). We do not represent this product as USP, BP, EP, or other pharmacopoeial grades.
How should ADA be handled safely?
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Handle ADA with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. Refer to the Safety Data Sheet for comprehensive handling information. WGK Germany is 3.
How is ADA packed, shipped, and exported?
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ADA is carefully packed to ensure stability during transit. Tech Serve Solutions exports chemicals globally, adhering to all relevant shipping regulations.
How can I request a sample or quote for ADA?
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To request a sample or a quotation for ADA, please contact our sales team through the website or by calling our main office.
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