7-Azido-4-Methylcoumarin
7-Azido-4-Methylcoumarin (CAS: 95633-27-5, C10H7N3O2, MW: 201.18) is a specialized fluorogenic probe for hydrogen sulfide (H2S) detection. Upon selective reduction by H2S, it generates the fluorescent 7-amino-4-methylcoumarin (AMC). This compound is valuable for monitoring enzymatic H2S production in vitro and visualizing H2S in cellular environments, offering high sensitivity and selectivity. It is readily soluble in DMSO.
Hydrogen Sulfide Detection
7-Azido-4-methylcoumarin serves as a sensitive and selective fluorogenic probe for detecting hydrogen sulfide (H2S). Its reaction with H2S produces fluorescent 7-amino-4-methylcoumarin (AMC), allowing for quantitative measurement of H2S levels.
Enzymatic H2S Monitoring
This probe is useful for monitoring the enzymatic production of H2S in various biological and chemical systems. It provides a quantifiable fluorescent signal correlating to the amount of H2S generated by enzyme activity.
Cellular H2S Visualization
7-Azido-4-methylcoumarin enables the visualization of H2S within cellular environments. The probe's fluorescence signal can be used to study the distribution and dynamics of H2S in living cells.
Chemical Biology Research
As a reagent in chemical biology, 7-Azido-4-methylcoumarin aids in the study of biological processes involving hydrogen sulfide. Its selectivity ensures reliable results in complex biological matrices.
| Molecular weight | 201.18 |
|---|---|
| Empirical formula | C10H7N3O2 |
| Application | 7-Azido-4-methylcoumarin (AzMC) is a fluorogenic probe useful for the detection of hydrogen sulfide (H2S). The aromatic azide moiety is selectively reduced in the presence of H2S, producing the fluorescent 7-amino-4-methylcoumarin (AMC) with a concomitant increase in fluorescence with ?ex = 365 nm and ?em = 450 nm. The probe can be used both to monitor enzymatic production of H2S in vitro 1 and also to visualize H2S in cells 2. The probe is highly sensitive and selective for H2S, providing a linear detection range in vitro between 200 nm – 100 μM H2S with 10 μM probe, and reacting selectively with H2S in the presence of mM concentrations of cysteine, homocysteine, glutathionine and many other common biological analytes. Caution: Use of this product with DTT, TCEP and/or biological thiols >25 mM should be avoided for maximum efficiency. AzMC is readily soluble in DMSO. |
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 7-Azido-4-Methylcoumarin primarily used for?
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7-Azido-4-Methylcoumarin (AzMC) is a fluorogenic probe primarily used for the sensitive and selective detection and visualization of hydrogen sulfide (H2S) in vitro and in cells.
What are the CAS number and molecular formula for 7-Azido-4-Methylcoumarin?
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The CAS number for 7-Azido-4-Methylcoumarin is 95633-27-5, and its molecular formula is C10H7N3O2.
What grade and purity does Tech Serve Solutions supply for 7-Azido-4-Methylcoumarin?
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Tech Serve Solutions supplies 7-Azido-4-Methylcoumarin for research purposes. The specific grade and purity are available upon request or by consulting the product's Certificate of Analysis. We do not represent this product as USP, BP, or EP grade.
What are the safety and handling considerations for 7-Azido-4-Methylcoumarin?
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7-Azido-4-Methylcoumarin (WGK Germany: 3) should be handled with appropriate personal protective equipment in a well-ventilated area. Avoid contact with skin, eyes, and clothing. Note that use with DTT, TCEP, and/or biological thiols exceeding 25 mM should be avoided for maximum efficiency.
Is 7-Azido-4-Methylcoumarin available for export and shipment?
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Yes, Tech Serve Solutions is a global supplier and exporter of fine chemicals, including 7-Azido-4-Methylcoumarin, and can facilitate international shipments.
How can I request a sample or quote for 7-Azido-4-Methylcoumarin?
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To request a sample or a quotation for 7-Azido-4-Methylcoumarin, please contact our sales department through the website or direct inquiry channels.
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