6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester
6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester (CAS: 114616-31-8; Formula: C31H26N2O10; MW: 586.55) is a reagent designed for the fluorescent labelling of amine-containing biomolecules. Its structure incorporates a fluorescein moiety, a six-carbon spacer, and an N-hydroxysuccinimide ester group, facilitating covalent conjugation. This compound is primarily utilised for introducing a fluorescent tag in biochemical and analytical applications, enhancing detection sensitivity.
- IUPAC
- Fluorescein-5(6)-carboxamidocaproic acid N-succinimidyl ester
![6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester (C31H26N2O10) — chemical structure, CAS 114616-31-8; Life Science, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F114616-31-8.png&w=3840&q=75)
Fluorescent Labelling
This reagent enables the introduction of the fluorescein fluorophore onto amine groups within biopolymers and other biomolecules. The hexanoic acid spacer offers increased accessibility of the reactive ester to nucleophiles, improving labelling efficiency.
Biochemical Research
Used in research settings to tag proteins, peptides, and other biomolecules for visualisation and quantification using fluorescence microscopy or spectroscopy. The fluorescein label provides bright and stable fluorescence.
Analytical Standards
This compound serves as a valuable tool in analytical chemistry, particularly in developing fluorescence-based detection methods. Its defined structure and fluorescent properties make it suitable for calibration and validation.
| Molecular weight | 586.55 |
|---|---|
| Empirical formula | C31H26N2O10 |
| Assay | ≥75% (HPLC) |
| Solubility | DMF: solublemethanol: soluble |
| Storage temperature | −20°C |
| Application | Reagent for introducing the fluorescein label; the spacer renders the reactive group more accessible to nucleophiles on biopolymers |
| Suitability | suitable for fluorescence |
Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 490 nm; λem 514 nm (pH 8.2)λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0 |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester used for?
+
It is used as a reagent for introducing the fluorescein label onto amine groups in biomolecules, facilitating fluorescent detection and analysis.
What are the CAS number and formula for this compound?
+
The CAS number is 114616-31-8, and the molecular formula is C31H26N2O10.
What grade and purity does Tech Serve Solutions supply?
+
Tech Serve Solutions supplies this product with an assay of ≥75% by HPLC. We do not represent this product as USP, BP, EP, or other pharmacopoeia grades.
How should this chemical be handled safely?
+
This compound is a Warning chemical, causing skin irritation (H315), serious eye irritation (H319), and respiratory irritation (H335). Handle with appropriate personal protective equipment, including a dust mask (type N95 US), eyeshields, and gloves. Avoid breathing dust and ensure adequate ventilation.
How is this product packed, shipped, and exported?
+
This chemical is carefully packed according to international shipping regulations to ensure safe transit. Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals.
How can I request a quotation or sample?
+
To request a quotation or a sample, please contact our sales team through the website or directly via email, providing the product name and CAS number.
▶ Related products
![(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (C8H13N3O4) — chemical structure, CAS 138472-01-2; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F138472-01-2.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide
C8H13N3O4
Biochemicals & Reagents![(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide (C14H18N4O4) — chemical structure, CAS 162626-99-5; Biochemicals & Reagents, fine chemical supplied by Tech Serve Solutions](/_next/image?url=https%3A%2F%2Fpub-f4d920c906024441a773a96f7098e551.r2.dev%2Fimages%2Fproducts%2F162626-99-5.png&w=3840&q=75)
(±)-(E)-4-Ethyl-2-[(Z)-hydroxyimino]-5-nitro-3-hexen-1-yl-nicotinamide
C14H18N4O4
Biochemicals & Reagents
(±)-2,5-Dimethoxy-4-bromoamphetamine hydrobromide
Biochemicals & Reagents
(±)-2-(p-Methoxyphenoxy)propionic acid
C10H12O4
Biochemicals & Reagents
(±)-2-Amino-6,7-dihydroxy-1,2,3,4-tetrahydronaphthalene hydrobromide
C10H13NO2·HBr
Biochemicals & Reagents
(±)-2-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxydecanoic acid
C10H20O3
Biochemicals & Reagents
(±)-3-Hydroxyoctanoic acid
C8H16O3
Biochemicals & Reagents▶ Explore more
3-Methyl-1,5-di-p-tolyl-1,4-pentazadiene
Chemical Synthesis
3-Methyl-1,5-pentanediol
Chemical Synthesis
3-Methyl-1-(2-methylphenyl)-1H-pyrazol-5-amine
C11H13N3
Chemical Synthesis
3-Methyl-1-(3′-sulfoamidophenyl)-5-pyrazolone
C10H11N3O3S
Chemical Synthesis