4-(4′-Hydroxyphenylazo)benzoic acid
4-(4′-Hydroxyphenylazo)benzoic acid (CAS: 105299-45-4; C13H10N2O3; MW: 242.23) is a functionalised azo compound primarily used as a cleavable linker in chemical biology and synthesis. Its azobenzene moiety can be selectively reduced, typically with sodium dithionite, to yield two primary amine functionalities, facilitating controlled release or separation. This property makes it valuable in proteomic studies and affinity chromatography.
- IUPAC
- (E)-4-[(4-Hydroxyphenyl)azo]benzoic acid
Reducible Linker
This compound functions as an azobenzene cleavable linker. Reduction of the azo bond, for instance with sodium dithionite, cleaves the molecule to release two primary amine groups.
Proteomics Research
The unique cleavable nature of 4-(4′-Hydroxyphenylazo)benzoic acid makes it suitable for applications in proteomics, aiding in the study of protein interactions and modifications.
Affinity Chromatography
It has been utilised in the development of affinity chromatography techniques, leveraging its specific cleavage properties for separation and purification processes.
Chemical Synthesis
As a bifunctional molecule, it serves as a versatile building block in organic synthesis, enabling the construction of complex molecular architectures.
| Molecular weight | 242.23 |
|---|---|
| Empirical formula | C13H10N2O3 |
| Assay | 97% |
| Storage temperature | 2-8°C |
| Application | Azobenzene cleavable linker. Treatment with sodium dithionite (sodium hydrosulfite) reduces azo functionality, cleaving the N-N bond to yield two primary amines.Has been used in proteomic and affinity chromatography applications. |
| Melting point | 270-280 °C |
Hazard statements
- H302Harmful if swallowed
- H319Causes serious eye irritation
Precautionary statements
- P305IF IN EYES
| Water hazard class (WGK, DE) | 3 |
|---|---|
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-36 |
| Safety statements (S) | 26 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 4-(4′-Hydroxyphenylazo)benzoic acid used for?
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It is primarily used as an azobenzene cleavable linker in chemical biology and synthesis. It facilitates controlled cleavage via reduction of the azo bond, yielding two primary amines, and has applications in proteomics and affinity chromatography.
What are the CAS number and molecular formula?
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The CAS number is 105299-45-4, and the molecular formula is C13H10N2O3.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 4-(4′-Hydroxyphenylazo)benzoic acid with an assay of 97%. This is a research-grade chemical and is not intended for use as USP, BP, EP, or pharmaceutical grade.
How should 4-(4′-Hydroxyphenylazo)benzoic acid be handled?
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Handle with appropriate personal protective equipment. It is harmful if swallowed (H302) and causes serious eye irritation (H319). In case of contact with eyes, rinse cautiously with water for several minutes, removing contact lenses if present and easy to do, and continue rinsing (P305 + P351 + P338). Store at 2-8°C.
How is this chemical packed, shipped, and exported?
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Tech Serve Solutions exports fine chemicals globally, adhering to all necessary regulations for safe and compliant packaging and shipment of laboratory reagents and intermediates.
How can I request a sample or quote?
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Please contact our customer service team through the website or by phone to request a sample or a detailed quotation for 4-(4′-Hydroxyphenylazo)benzoic acid.
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