3,5-Dinitrosalicylic acid
3,5-Dinitrosalicylic acid (CAS: 609-99-4) is a dinitrated derivative of salicylic acid with the molecular formula C7H4N2O7 and a molecular weight of 228.12 g/mol. This chemical compound is primarily utilised as a laboratory reagent. Its applications include the preparation of oxazolines, spectrophotometric determination of ampicillin, and the measurement of silver nanoparticle effects on reducing sugars.
- IUPAC
- 3,5-Dinitro-2-hydroxybenzoic acid,DNS
- Synonyms
- (O2N)2C6H2-2-(OH)CO2H3,5-Dinitro-2-hydroxybenzoic acidDNS

Preparation of Oxazolines
3,5-Dinitrosalicylic acid serves as a reagent in the synthesis of oxazoline compounds from amino alcohols, a class of heterocyclic molecules with diverse applications in organic chemistry.
Spectrophotometric Determination of Ampicillin
This compound is employed as a reagent in analytical chemistry for the spectrophotometric quantification of ampicillin, a widely used antibiotic.
Assay of Reducing Sugars
3,5-Dinitrosalicylic acid is used in biochemical assays to measure the concentration of reducing sugars, and to study the impact of substances like silver nanoparticles on carbohydrate metabolism.
| Molecular weight | 228.12 |
|---|---|
| Linear formula | (O2N)2C6H2-2-(OH)CO2H |
| Assay | 98% |
| Solubility | H2O: soluble50 mg/mL |
| Application | 3,5-Dinitrosalicylic acid was used as a reagent for the preparation of oxazolines from amino alcohols1 and for the spectrophotometric determination of ampicillin2. It was also used to measure the effects of silver nanoparticles on the membrane leakage of the reducing sugars3. |
| Melting point | 168-172 °C(lit.) |

Hazard statements
- H302Harmful if swallowed
- H315Causes skin irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xn |
| Risk statements (R) | 22-37/38 |
| Safety statements (S) | 22-24/25 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 3,5-Dinitrosalicylic acid used for?
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3,5-Dinitrosalicylic acid is used as a laboratory reagent for applications such as the preparation of oxazolines, the spectrophotometric determination of ampicillin, and measuring the effects of silver nanoparticles on the membrane leakage of reducing sugars.
What is the CAS number and chemical formula for 3,5-Dinitrosalicylic acid?
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The CAS number for 3,5-Dinitrosalicylic acid is 609-99-4. Its linear formula is (O₂N)₂C₆H₂-2-(OH)CO₂H, and its molecular weight is 228.12 g/mol.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 3,5-Dinitrosalicylic acid with an assay of 98%. We do not represent this product as USP, BP, EP, or any pharmacopoeia grade; it is suitable for laboratory and research purposes.
What are the safety and handling precautions for 3,5-Dinitrosalicylic acid?
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3,5-Dinitrosalicylic acid is classified with a Warning signal word and carries hazard statements H302 (Harmful if swallowed), H315 (Causes skin irritation), and H335 (May cause respiratory irritation). Users should wear a dust mask (N95 US type), eyeshields, and gloves. Avoid breathing dust (P261).
Is 3,5-Dinitrosalicylic acid a controlled substance?
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Based on the provided data, 3,5-Dinitrosalicylic acid (CAS: 609-99-4) is not indicated as a controlled substance. Safety information includes hazard codes Xn and risk statements 22-37/38.
How is 3,5-Dinitrosalicylic acid packed and shipped?
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Tech Serve Solutions packs and exports fine chemicals, pharmaceutical intermediates, and laboratory reagents globally, adhering to international shipping regulations for chemical products.
How can I request a sample or quote for 3,5-Dinitrosalicylic acid?
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To request a sample or a quotation for 3,5-Dinitrosalicylic acid, please contact our sales department through the TSS website or by calling our customer service line.
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