2-Nitrophenyl β-D-galactopyranoside

CAS 369-07-3C12H15NO8Biochemicals & Reagents

2-Nitrophenyl β-D-galactopyranoside (CAS 369-07-3), with the molecular formula C12H15NO8 and a molecular weight of 301.25, is a key chromogenic substrate. It is widely employed in biochemical research and diagnostics for the detection and quantification of β-galactosidase activity. This compound enables visible colour changes upon enzymatic hydrolysis, facilitating enzyme assays and microbial identification.

IUPAC: o-Nitrophenyl β-D-galactopyranoside,ONPG
Synonyms: o-Nitrophenyl β-D-galactopyranoside, ONPG

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2-Nitrophenyl β-D-galactopyranoside (C12H15NO8) — chemical structure, CAS 369-07-3; Life Science, fine chemical supplied by Tech Serve Solutions

Biochemicals and ReagentsEnzyme SubstratesEnzymesInhibitorsand SubstratesGalactosidasebetaSubstrates by Enzyme

01 /Applications

Enzyme Assays for β-Galactosidase

Utilised as a chromogenic substrate to detect and quantify the activity of the enzyme β-galactosidase. Enzymatic cleavage releases a coloured product, allowing for straightforward visual or spectrophotometric monitoring.

Microbiological Identification

Serves as a diagnostic tool in microbiology for identifying organisms possessing β-galactosidase activity. This is particularly useful in differentiating bacterial species in clinical and environmental samples.

Biochemical Research

A standard reagent in molecular biology and biochemistry laboratories for studying enzyme kinetics and mechanisms related to glycosidases.

02 /Properties

Molecular weight	301.25
Empirical formula	C12H15NO8
Assay	≥99.0% (HPLC)
Optical activity	[α]20/D −70±1°, c = 1% in H2O
Storage temperature	2-8°C

03 /Safety & handling

Protective equipment	Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Water hazard class (WGK, DE)	3

Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.

04 /Identifiers & registry

CAS number

369-07-3

MDL number

MFCD00063255

PubChem substance

24886478

Beilstein registry

92207

05 /Quality & supply

Documentation

Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.

Supply & logistics

Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.

06 /Frequently asked questions

What is 2-Nitrophenyl β-D-galactopyranoside used for?

2-Nitrophenyl β-D-galactopyranoside is primarily used as a chromogenic substrate for the detection and quantification of β-galactosidase activity in biochemical research and microbiological identification.

What is the CAS number and chemical formula for 2-Nitrophenyl β-D-galactopyranoside?

The CAS number is 369-07-3 and the empirical formula (Hill notation) is C12H15NO8.

What grade and purity does Tech Serve Solutions supply?

Tech Serve Solutions supplies 2-Nitrophenyl β-D-galactopyranoside with an assay of ≥99.0% (HPLC). This product is intended for research and laboratory use and is not represented as USP, BP, EP, or other pharmacopoeial grades.

How should 2-Nitrophenyl β-D-galactopyranoside be handled safely?

Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 (US) or type P1 (EN143) respirator filter is recommended. The compound carries a WGK Germany rating of 3, indicating it is hazardous to water.

How is this chemical shipped and exported?

Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals and reagents, adhering to all necessary regulations for international shipping.