1-Methyl-3-indolyl-β-D-galactopyranoside
1-Methyl-3-indolyl-β-D-galactopyranoside (CAS: 207598-26-3) is a chromogenic substrate with the molecular formula C15H19NO6 and a molecular weight of 309.31 g/mol. This biochemical reagent is specifically designed for the detection and quantification of β-galactosidase activity. Upon enzymatic cleavage, it yields a characteristic green insoluble product, making it invaluable in molecular biology and biochemical research for various assay applications.
- IUPAC
- Green-β-D-gal,N-Methylindolyl-β-D-galactopyranoside

Chromogenic Detection of β-Galactosidase
Utilised as a sensitive chromogenic substrate, this compound allows for the visual detection of β-galactosidase activity. The enzyme cleaves the galactoside bond, releasing an indolyl derivative that precipitates as a green-coloured product.
Enzyme Assays and Research
Its primary application lies in biochemical and molecular biology research for quantifying β-galactosidase levels in various biological samples. This is crucial for applications such as reporter gene assays and screening for enzyme activity.
Glycobiology and Proteomics
Serves as a valuable tool in glycobiology and proteomics studies, particularly those involving post-translational modifications or enzyme characterisation related to galactosidase function.
| Molecular weight | 309.31 |
|---|---|
| Empirical formula | C15H19NO6 |
| Assay | ≥98.0% (HPLC) |
| Optical activity | [α]20/D −41.5±2°, c = 1% in DMF/H2O 1:1 |
| Storage temperature | 2-8°C |
| Application | Chromogenic substrate for β-galactosidase producing a green insoluble product. |
| Protective equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
|---|---|
| Water hazard class (WGK, DE) | 3 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 1-Methyl-3-indolyl-β-D-galactopyranoside used for?
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This compound is used as a chromogenic substrate for the detection and quantification of β-galactosidase activity, producing a green insoluble product upon enzymatic cleavage.
What are the CAS number and formula for 1-Methyl-3-indolyl-β-D-galactopyranoside?
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The CAS number is 207598-26-3 and the empirical formula is C15H19NO6.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies 1-Methyl-3-indolyl-β-D-galactopyranoside with an assay of ≥98.0% (HPLC). This product is intended for research purposes and is not supplied as USP, BP, EP, or pharmacopoeia grade.
How should 1-Methyl-3-indolyl-β-D-galactopyranoside be handled and stored?
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Handle with appropriate personal protective equipment, including eyeshields and gloves. A type N95 or P1 respirator filter is recommended. Store at 2-8°C to maintain stability.
Is this chemical shipped internationally by TSS?
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Yes, Tech Serve Solutions is a specialist global supplier and exporter of fine chemicals, including 1-Methyl-3-indolyl-β-D-galactopyranoside.
How can I request a sample or quote for this chemical?
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Please contact our sales team via the website or phone to request a sample or obtain a quotation for 1-Methyl-3-indolyl-β-D-galactopyranoside.
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