1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid
1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (CAS: 85233-19-8), with the molecular formula C22H24N2O10, is a highly selective calcium chelating reagent. It is crucial for the spectrophotometric monitoring of Ca<sup>2+</sup> levels in various analytical applications. This compound functions by forming stable complexes with calcium ions, enabling accurate detection and quantification. It is a valuable tool in biochemical research and diagnostics.
- IUPAC
- BAPTA,Ethylenedioxybis(o-phenylenenitrilo)tetraacetic acid
- Synonyms
- BAPTAEthylenedioxybis(o-phenylenenitrilo)tetraacetic acid

Calcium Ion Monitoring
This reagent is specifically designed for the highly selective chelation of calcium ions. Its properties make it suitable for the spectrophotometric monitoring of Ca<sup>2+</sup> concentrations in biological and chemical samples.
Spectrophotometric Analysis
Utilised in spectrophotometric methods, this compound aids in the accurate determination of calcium ion levels. It forms complexes with Ca<sup>2+</sup> that can be measured spectroscopically.
Biochemical Research
As a specialized chelator, it finds application in biochemical research where precise control or measurement of calcium ion activity is required. It can be employed in studies involving cellular signalling or enzyme kinetics.
| Molecular weight | 476.43 |
|---|---|
| Empirical formula | C22H24N2O10 |
| Assay | ≥96.0% (HPLC) |
| Storage temperature | 2-8°C |
| Application | Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels |

Hazard statements
- H315Causes skin irritation
- H319Causes serious eye irritation
- H335May cause respiratory irritation
Precautionary statements
- P261Avoid breathing dust, fume, gas or vapours
- P305IF IN EYES
| Protective equipment | dust mask type N95 (US), Eyeshields, Gloves |
|---|---|
| Water hazard class (WGK, DE) | 3 |
| Hazard codes (EU) | Xi |
| Risk statements (R) | 36/37/38 |
| Safety statements (S) | 26-36 |
Hazard information is provided for guidance. Always consult the product Safety Data Sheet (SDS), available on request, before handling.
| Fluorescence | λex 251 nm; λem 363 nm (pH 8.0 Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels)λex 274 nm; λem 363 nm (With Calcium) |
|---|
Documentation
Every batch ships with a Certificate of Analysis covering assay, identity and purity; the grade is confirmed against your enquiry. Safety Data Sheets and technical data sheets are available on request.
Supply & logistics
Samples for technical evaluation; bulk MOQ by grade and packaging. In-stock material ships in 7–10 working days, worldwide, with full export documentation.
What is 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid used for?
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This compound is a highly selective calcium chelating reagent, primarily used for the spectrophotometric monitoring of Ca<sup>2+</sup> levels in various analytical and research applications.
What are the CAS number and chemical formula for this product?
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The CAS number is 85233-19-8, and the empirical formula (Hill notation) is C22H24N2O10.
What grade and purity does Tech Serve Solutions supply?
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Tech Serve Solutions supplies this product with an assay of ≥96.0% (HPLC). We do not represent this product as USP, BP, EP, or any other pharmacopoeial grade unless explicitly stated in the product specifications.
How should 1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid be handled safely?
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This compound is a warning irritant (H315, H319, H335). Handle with appropriate personal protective equipment, including a dust mask (type N95), eyeshields, and gloves. Avoid dust formation and inhalation, and ensure good ventilation.
How is this chemical packed, shipped, and exported?
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Tech Serve Solutions, as a specialist global supplier and exporter of fine chemicals, ensures appropriate packaging for shipment and export, adhering to all relevant regulations.
How can I request a sample or quote for this product?
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To request a sample or a quotation, please contact our sales team through the provided channels on the Tech Serve Solutions website.
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